Generator

Part:BBa_K1361003

Designed by: Shoujie Sun   Group: iGEM14_Tianjin   (2014-10-06)


Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C

This part contains the major subunit of curli fiber CsgA and its nucleator CsgB, in which CsgA is constitutive expression(a relatively strong promoter compared to BBa_K1361001) whereas CsgB is under the control of T7 promotor. This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 646
    Illegal NheI site found at 669
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None